Class 2. Study Guide

Topic:

Path 1. Nucleic acids: levels of DNA compactification. Methods for studying the structure and functions of nucleic acids.

Path 2. DNA replication and repair: mechanisms and biomedical significance. DNA structure damage and repair mechanisms. Medicine that inhibit replication.

Study questions:

  1. Levels of structural organization of chromatin. The role of histone and non-histone proteins in DNA compaction.
  2. Methods for studying the structure and functions of nucleic acids.
  3. DNA replication: basic principles of matrix biosynthesis. Consistency of replication and cell cycle.
  4. Initiation of DNA replication: the scheme of the process, the main enzymes and their function.
  5. Elongation and termination of DNA replication: the scheme of the process, the main enzymes and their function. Mechanisms of biosynthesis of leading and lagging DNA chains.
  6. Mechanisms of DNA structure damage and their biological consequences.
  7. DNA repair: the main mechanisms of DNA structure restoration. Violations of DNA repair systems and their biomedical significance.
  8. Medicine that inhibit replication.

Vocabulary (list of mandatory terms and concepts):

  • nucleoside;
  • nucleic acids;
  • hydrogen bonding;
  • biopolymers;
  • Chargaff rule;
  • histones;
  • the nucleosome;
  • euchromatin;
  • heterochromatin;
  • apurinization;
  • interphase;
  • replication origin;
  • replicon;
  • photolyase;
  • DNA insertase;
  • semi - conservativeness;
  • replication fork;
  • fragments of Okazaki;
  • ligation;
  • telomerase;
  • Hayflick limit.
Последнее изменение: Вторник, 6 Сентябрь 2022, 20:16