Class 9. Study Guide

Topic:

Principles of coordination of metabolic pathways. Regulation of enzymatic activity. Proteins and enzymes as targets for drugs.

Study questions:

  1. Metabolic pathways and the biological significance of their regulation.
  2. Principles of regulation of enzyme activity.
  3. Examples and functional role of cofactors – metal ions.
  4. Examples and functional role of cofactors – vitamin derivatives.
  5. Allosteric regulation of enzymatic activity.
  6. Inhibitors of enzymatic activity: classification and mechanisms of action.
  7. Regulation of enzyme activity by protein-protein interactions.
  8. Adenylate cyclase mechanism of hormonal signal transmission. The role of cAMP.
  9. Regulation of enzyme activity by covalent modification. Phosphorylation and dephosphorylation.
  10. Partial proteolysis: biological significance and examples.
  11. Principles of using enzymes as clinical and laboratory biomarkers.
  12. Diagnostic value of isoenzymes.
  13. Limitations of determining the activity of enzymes for the diagnosis of diseases.
  14. Enzymes as medicines.

Vocabulary (list of mandatory terms and concepts):

  • metabolic pathway,
  • enzyme lability,
  • efficiency of enzymes,
  • catalytic specificity of enzymes,
  •  prosthetic groups,
  • Michaelis constant,
  • allosteric effectors of enzymes,
  • zymogens,
  • partial proteolysis,
  • G-proteins,
  • adenylate cyclase,
  • protein kinases,
  • phosphatases,
  • heterotropic regulation,
  • homotropic regulation,
  • competitive inhibition,
  • non-competitive inhibition;
Последнее изменение: Пятница, 21 Октябрь 2022, 08:43